Technical Reference #595

595.

Cellular Adaptation To Mechanical Stress: Role Of Integrins; Rho; Cytoskeletal Tension And Mechanosensitive Ion Channels Benjamin D. Matthews; Darryl R. Overby; Robert Mannix and Donald E. Ingber, Children's Hospital Boston, The Journal of Cell Science, 119(595), (2006)
Link To Paper

Abstract:
To understand how cells sense and adapt to mechanical stress we applied tensional forces to magnetic microbeads bound to cell-surface integrin receptors and measured changes in bead displacement with sub-micrometer resolution using optical microscopy. Ce

Keywords:
Integrin; Focal adhesion; Mechanotransduction; Prestress; Tension; Magnetometry

Materials & Methods:
In preparation for experiments cells were cultured in DMEM with 0.5% FCS for 24 hours trypsinized (Trypsin-EDTA Gibco) and then plated (1.5 104 cells/dish) onto glass-bottomed 35 mm dishes (MatTek Corp)…

Microscopic Technique
Optical Microscopy

Cell Type(s)
Capillary Endothelial