Technical Reference #595

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed 35 mm dishes (MatTek Corp)

595.

Cellular Adaptation To Mechanical Stress: Role Of Integrins, Rho, Cytoskeletal Tension And Mechanosensitive Ion Channels Benjamin D. Matthews, Darryl R. Overby, Robert Mannix and Donald E. Ingber, Children's Hospital Boston, The Journal of Cell Science, 119(3), 516, (2006)
Link To Paper

Abstract:
To understand how cells sense and adapt to mechanical stress, we applied tensional forces to magnetic microbeads bound to cell-surface integrin receptors and measured changes in bead displacement with sub-micrometer resolution using optical microscopy. Ce

Keywords:
Integrin, Focal adhesion, Mechanotransduction, Prestress, Tension, Magnetometry

Materials & Methods:
In preparation for experiments, cells were cultured in DMEM with 0.5% FCS for 24 hours, trypsinized (Trypsin-EDTA, Gibco), and then plated (1.5 104 cells/dish) onto glass-bottomed 35 mm dishes (MatTek Corp)�

Microscopic Technique
Optical Microscopy

Cell Type(s)
Capillary Endothelial