Technical Reference #594

594.

Disruption Of Nucleocytoplasmic Trafficking Of Cyclin D1 And Topoisomerase Ii By Sanguinarine Jon Holy, Genelle Lamont and Edward Perkins, University of Minnesota, BMC Cell Biology, 7(13), 10, (2006)
Link To Paper

Abstract:
The quaternary isoquinoline alkaloid sanguinarine is receiving increasing attention as a potential chemotherapeutic agent in the treatment of cancer.

Keywords:
breast cancer, sanguinarine, DNA synthesis

Materials & Methods:
Cells were seeded into 35 mm glass-bottom Petri dishes (MatTek Inc., Amherst, MA) at a density of 2.5 × 104/ml, and allowed to recover for 2 days prior to use.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MCF-7