Technical Reference #592

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed culture dishes (MatTek, Ashland, MA)

592.

Atp Stimulates Grk-3 Phosphorylation And -Arrestin-2-Dependent Internalization Of P2X7 Receptor Ying-Hong Feng, Liqin Wang, Qifang Wang, Xin Li, Robin Zeng, and George I. Gorodeski, Uniformed Services University of the Health Sciences, American Journal of Physiology: Cell Physiology, 288(6), C1344, (2005)
Link To Paper

Abstract:
The objective of this study was to understand the mechanisms involved in P2X7 receptor activation.

Keywords:
purinergic receptor, recycling, dynamin, clathrin, cervix, epithelium

Materials & Methods:
CaSki cells transfected with -arrestin-2-GFP or HEK-293 cells cotransfected with the full-length human P2X7 receptor and -arrestin-2-GFP were plated on 35-mm glass-bottomed culture dishes (MatTek, Ashland, MA).

Microscopic Technique
Light Microscopy, Confocal

Cell Type(s)
HEK-293