Technical Reference #591

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed culture dishes (MatTek, Ashland, MA)

591.

Actin And Alpha-Actinin Dynamics In The Adhesion And Motility Of Epec And Ehec On Host Cells Nathan C. Shaner, Joseph W. Sanger, and Jean M. Sanger, University of Pennsylvania, Cell Motility and the Cytoskeleton, 60(2), 106, (2005)
Link To Paper

Abstract:
Two pathogenic Escherichia coli, Enteropathogenic E. coli (EPEC) and Enterohemorrhagic E. coli (EHEC), adhere to the outside of host cells and induce cytoskeletal rearrangements leading to the formation of membrane-encased pedestals comprised of actin filaments and other associated proteins beneath the bacteria.

Keywords:
actin, alpha-actinin, EPEC, EHEC, FRAP, pathogens

Materials & Methods:
PtK2 cells were grown on 35-mm glass-bottom dishes (MatTek, Ashland, MA) as described by Ayoob et al. [2000b].

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Ptk2