Technical Reference #591

591.

Actin And Alpha-Actinin Dynamics In The Adhesion And Motility Of Epec And Ehec On Host Cells Nathan C. Shaner; Joseph W. Sanger; and Jean M. Sanger, University of Pennsylvania, Cell Motility and the Cytoskeleton, 60(591), (2005)
Link To Paper

Abstract:
Two pathogenic Escherichia coli Enteropathogenic E. coli (EPEC) and Enterohemorrhagic E. coli (EHEC) adhere to the outside of host cells and induce cytoskeletal rearrangements leading to the formation of membrane-encased pedestals comprised of actin filaments and other associated proteins beneath the bacteria.

Keywords:
actin; alpha-actinin; EPEC; EHEC; FRAP; pathogens

Materials & Methods:
PtK2 cells were grown on 35-mm glass-bottom dishes (MatTek Ashland MA) as described by Ayoob et al. [2000b].

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Ptk2