Technical Reference #588

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm glass-bottom tissue culture dishes (MatTek Corporation, MA)

588.

Mechanisms Of Cx43 And Cx26 Transport To The Plasma Membrane And Gap Junction Regeneration Tamsin Thomas, Karen Jordan, Jamie Simek, Qing Shao, Chris Jedeszko, Paul Walton and Dale W. Laird, University of Western Ontario, The Journal of Cell Science, 118(19), 4452, (2005)
Link To Paper

Abstract:
Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family.

Keywords:
Connexin, Gap junctions, Post-Golgi carriers, Timelapse imaging, Fluorescence recovery after photobleaching

Materials & Methods:
BICR-M1Rk cells transiently expressing Cx43-GFP or stably expressing Cx26-YFP were plated in 35 mm glass-bottom tissue culture dishes for live imaging (MatTek Corporation, MA).

Microscopic Technique
Confocal Microscopy, Inverted confocal microscopy

Cell Type(s)
BICR-M1Rk