Technical Reference #588

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm glass-bottom tissue culture dishes (MatTek Corporation; MA)

588.

Mechanisms Of Cx43 And Cx26 Transport To The Plasma Membrane And Gap Junction Regeneration Tamsin Thomas; Karen Jordan; Jamie Simek; Qing Shao; Chris Jedeszko; Paul Walton and Dale W. Laird, University of Western Ontario, The Journal of Cell Science, 118(588), (2005)
Link To Paper

Abstract:
Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family.

Keywords:
Connexin; Gap junctions; Post-Golgi carriers; Timelapse imaging; Fluorescence recovery after photobleaching

Materials & Methods:
BICR-M1Rk cells transiently expressing Cx43-GFP or stably expressing Cx26-YFP were plated in 35 mm glass-bottom tissue culture dishes for live imaging (MatTek Corporation MA).

Microscopic Technique
Confocal Microscopy, Inverted confocal microscopy

Cell Type(s)
BICR-M1Rk