Technical Reference #585

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
dishes containing glass bottoms (MatTek Corp.; 1.5 ·105 cells per dish)

585.

Ligand-Selective Targeting Of The Glucocorticoid Receptor To Nuclear Subdomains Is Associated With Decreased Receptor Mobility Marcel J.M. Schaaf; Laura J. Lewis-Tuffin and John A. Cidlowski, National Institutes of Health, Molecular Endocrinology, 19(585), (2005)
Link To Paper

Abstract:
The association between nuclear distribution and mobility of the human glucocorticoid receptor was examined in living COS-1 cells using YFP- and CFP-tagged receptors. Quantitation of the nuclear distribution induced by an array of glucocorticoid ligands r

Keywords:
glucocorticoid receptor; nuclear distribution; steroid hormone; FRAP

Materials & Methods:
One day after transfection cells were transferred to 9.6 cm2 dishes containing glass bottoms (MatTek Corp. 1.5 ·105 cells per dish).

Microscopic Technique
Confocal Microscopy, Time Lapse

Cell Type(s)
COS-1