Technical Reference #584

584.

Characterization Of The Myosin-Based Source For Second-Harmonic Generation From Muscle Sarcomeres. Sergey V. Plotnikov; Andrew C. Millard; Paul J. Campagnola; and William A. Mohler, University of Connecticut, Biophysical Journal, 90(584), (2006)
Link To Paper

Abstract:
Several biologically important protein structures give rise to strong second-harmonic generation (SHG) in their native context. In addition to high-contrast optical sections of cells and tissues SHG imaging can provide detailed structural information bas

Keywords:
second-harmonic; generation (SHG); sarcomere; myosin; striated muscle; SHG harmonophore

Materials & Methods:
An aliquot (0.5 ml) of isolated scallop myofibril suspension was placed into a glass bottom Petri dish (MatTek Corp. Ashland MA) and the dish was centrifuged at 4000g for 10 min.

Microscopic Technique
Optical Microscopy, Nonlinear

Cell Type(s)
Myofibrils