Technical Reference #583

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm culture dish that incorporated a no. 1.5 glass coverslip-sealed 15-mm cut-out on the bottom (MatTek; Ashland; MA)

583.

Postprenylation Caax Processing Is Required For Proper Localization Of Ras But Not Rho Gtpases David Michaelson; Wasif Ali; Vi K. Chiu; Martin Bergo; Joseph Silletti; Latasha Wright; Stephen G. Young; and Mark Philips, New York University, Molecular Biology of the Cell, 16(583), (2005)
Link To Paper

Abstract:
The CAAX motif at the C terminus of most monomeric GTPases is required for membrane targeting because it signals for a series of three posttranslational modifications that include isoprenylation endoproteolytic release of the C-terminal– AAX amino acids

Keywords:
CAAX motif; membrane targeting; amino acids; isoprenylcysteine; prenylcysteine; actin remodeling

Materials & Methods:
For all microscopycells were plated transfected and imaged in the same 35-mm culture dish that incorporated a no. 1.5 glass coverslip-sealed 15-mm cut-out on the bottom (MatTek Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MEF