Technical Reference #582

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-coated 35 mm dishes (Mattek)

582.

Analysis Of Nuclear Transport Signals In The Human Apurinic/Apyrimidinic Endonuclease (Ape1/Ref1) Elias B. Jackson; Corey A. Theriot; Ranajoy Chattopadhyay; Sankar Mitra and Tadahide Izumi, University of Texas, Nucleic Acids Research, 33(582), (2005)
Link To Paper

Abstract:
Themammalianabasic-endonuclease1/redox-factor1 (APE1/Ref1) is an essential protein whose subcellular distributiondependsonthe cellular physiological status.

Keywords:
abasic-endonuclease1/redox-factor1 (APE1/Ref1); reactive oxygen species; gene regulation; nuclear transport; Karyopherin a; amino acid

Materials & Methods:
For live cell analysis cells spread on glass-coated 35 mm dishes (Mattek) were transfected with DNA as described above and then examined without fixation.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
BALB/c