Technical Reference #581

581.

Gi And G Subunits Both Define Selectivity Of G Protein Activation By 2-Adrenergic Receptors Scott K. Gibson and Alfred G. Gilman, University of Texas Southwestern Medical Center, PNAS, 103(581), (2006)
Link To Paper

Abstract:
Previous studies of the specificity of receptor interactions with G protein subunits in living cells have relied on measurements of second messengers or other downstream responses.

Keywords:
fluorescence resonance energy transfer; GTP-binding protein; subunits; GTP-binding protein; subunits; small interfering RNA

Materials & Methods:
Five hours after transfection cells were split into four 35-mm glass bottom culture dishes (MatTek).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa