Technical Reference #580

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen coated glass bottom 35 mm dishes (MatTek; Ashland; MA)

580.

Targeting Of Voltage-Gated Potassium Channel Isoforms To Distinct Cell Surface Microdomains Kristen M. S. O’Connell and Michael M. Tamkun, Colorado State University, The Journal of Cell Science, 118(580), (2005)
Link To Paper

Abstract:
Voltage-gated potassium (Kv) channels regulate action potential duration in nerve and muscle; therefore changes in the number and location of surface channels can profoundly influence electrical excitability.

Keywords:
Kv channels; Membrane trafficking; Lipid rafts

Materials & Methods:
For live cell imaging HEK cells were grown in 60 mm culture dishes and 24 hours post-transfection were passed at a 1:4 dilution onto collagen coated glass bottom 35 mm dishes (MatTek Ashland MA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293