Technical Reference #579

579.

A Carboxyl Leucine-Rich Region Of Parathyroid Hormone- Related Protein Is Critical For Nuclear Export Jared C. Pache, Douglas W. Burton, Leonard J. Deftos, and Randolph H. Hastings, University of California, San Diego (UCSD), Veterans Affairs San Diego Healthcare System, San Diego, California, Endocrinology, 147(2), 991, (2006)
Link To Paper

Abstract:
Parathyroid hormone-related protein is an oncofetal protein with distinct proliferative and anti-apoptotic roles that are affected by nucleocytoplasmic shuttling.

Keywords:
consensus sequence, confocal microscopy, exportin 1, fluorescence recovery after photobleaching, leptomycin, nucleocytoplasmic transport proteins, peptide hormones

Materials & Methods:
To assess steady state cellular distributions of PTHrP, 293 cells were plated in 35-mm glass bottom culture dishes (MatTek, Ashland, MA) and transfected with GFP3-PTHrP chimeras.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293