Technical Reference #579

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom culture dishes (MatTek; Ashland; MA)

579.

A Carboxyl Leucine-Rich Region Of Parathyroid Hormone- Related Protein Is Critical For Nuclear Export Jared C. Pache; Douglas W. Burton; Leonard J. Deftos; and Randolph H. Hastings, University of California San Diego (UCSD), Endocrinology, 147(579), (2006)
Link To Paper

Abstract:
Parathyroid hormone-related protein is an oncofetal protein with distinct proliferative and anti-apoptotic roles that are affected by nucleocytoplasmic shuttling.

Keywords:
consensus sequence; confocal microscopy; exportin 1; fluorescence recovery after photobleaching; leptomycin; nucleocytoplasmic transport proteins; peptide hormones

Materials & Methods:
To assess steady state cellular distributions of PTHrP 293 cells were plated in 35-mm glass bottom culture dishes (MatTek Ashland MA) and transfected with GFP3-PTHrP chimeras.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293