Technical Reference #578

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen-coated, glass bottom 35-mm tissue culture dishes (Mat-Tek, Ashland, MA)

578.

Apoptotic Membrane Blebbing Is Regulated By Myosin Light Chain Phosphorylation Jason C. Mills, Nicole L. Stone, Joseph Erhardt, and Randall N. Pittman, University of Pennsylvania, The Journal of Cell Biology, 140(3), 629, (1998)
Link To Paper

Abstract:
The evolutionarily conserved execution phase of apoptosis is defined by characteristic changesoccurring during the final stages of death; specificallycell shrinkage, dynamic membrane blebbing, condensationof chromatin, and DNA fragmentation. Mechan

Keywords:
membrane blebbing, apoptosis, z-VAD-FMK, myosin light chain kinase inhibitors, cell contraction, execution phase

Materials & Methods:
For Hoechst staining after serum removal or staurosporine treatment, 300,000 cells were plated on collagen-coated, glass bottom 35-mm tissue culture dishes (Mat-Tek, Ashland, MA). After experimental treatments, cells were rinsed three times with PBS

Microscopic Technique
Video Microscopy, Time Lapse

Cell Type(s)
PC12