Technical Reference #578

578.

Apoptotic Membrane Blebbing Is Regulated By Myosin Light Chain Phosphorylation Jason C. Mills; Nicole L. Stone; Joseph Erhardt; and Randall N. Pittman, University of Pennsylvania, The Journal of Cell Biology, 140(578), (1998)
Link To Paper

Abstract:
The evolutionarily conserved execution phase of apoptosis is defined by characteristic changesoccurring during the final stages of death; specificallycell shrinkage dynamic membrane blebbing condensationof chromatin and DNA fragmentation. Mechan

Keywords:
membrane blebbing; apoptosis; z-VAD-FMK; myosin light chain kinase inhibitors; cell contraction; execution phase

Materials & Methods:
For Hoechst staining after serum removal or staurosporine treatment 300000 cells were plated on collagen-coated glass bottom 35-mm tissue culture dishes (Mat-Tek Ashland MA). After experimental treatments cells were rinsed three times with PBS

Microscopic Technique
Video Microscopy, Time Lapse

Cell Type(s)
PC12