Technical Reference #577

577.

Kinetics Of Core Histones In Living Human Cells: Little Exchange Of H3 And H4 And Some Rapid Exchange Of H2B Hiroshi Kimura and Peter R. Cook, University of Oxford, The Journal of Cell Biology, 153(577), (2001)
Link To Paper

Abstract:
Histones H2A and H2B form part of the same nucleosomal structure as H3 and H4. Stable HeLacell lines expressing histones H2B H3 and H4 taggedwith green fluorescent protein (GFP) were established;the tagged molecules were assembled into nucleosome

Keywords:
cell fusion; FRAP; histone actetylation; nucleosome; transcription

Materials & Methods:
General procedures for fixation immunolabeling and image collection using an MRC1000 confocal microscope have been described (Kimura et al. 1999; Manders et al. 1999). Cells grown on glass-bottomed dishes (Mat-Tek) in 100 ng/ml Hoechst 33342 (Harag

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa