Technical Reference #574

574.

Rapid Suppression Of Free Radical Formation By Nerve Growth Factor Involves The Mitogen-Activated Protein Kinase Pathway LAURA L. DUGAN; DOUGLAS J. CREEDON; EUGENE M. JOHNSON; JR.; AND DAVID M. HOLTZMAN, Washington University, PNAS, 94(574), (1997)
Link To Paper

Abstract:
Neurotrophins such as nerve growth factor(NGF) regulate neuronal survival during development andare neuroprotective in certain models of injury to both theperipheral and the central nervous system. Although manyeffects of neurotrophins involve lon

Keywords:
nerve growth factor; protein kinase pathway; neurotrophins; neuronal death

Materials & Methods:
For imaging suspensions of GT1-1 or GT1-1 trk cells were diluted and plated onto 35-mm culture dishes (Mat-Tek Ashland MA) possessing an oval cut-out sealed by a glass coverslip coated with poly-D-lysine: laminin (12). Cultures were analyzed when

Microscopic Technique
Confocal Microscopy

Cell Type(s)
GT1-1