Technical Reference #572

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Plaste k M c ell culture plates were from Mat Tek Co. (Ashland; MA)

572.

Effect Of Mast Cell Granules On The Gene Expression Of Nitric Oxide Synthase And Tumour Necrosis Factor-A In Macrophages Y. Li; T. D. Nguyen; A. C. Stechschulte;D. J. Stechschulte and K. N. Dileepan, University of Kansas, Mediators of Inflammation, 7(572), (1998)
Link To Paper

Abstract:
Previous studies have shown that mas t cell granule s(MCG) inhibit nume rous macrophage functionsincluding tumour cytotox ic ity supe rox ide and nitricox ide (NO) production and FCg 2a re cepto r-mediatedphagocytos is . In th is study the e

Keywords:
Nitric oxide; TNFa ; Macrophages; Mast cell granules; Proteases

Materials & Methods:
Lipopolysaccharide (LPS) from Es che richia co li ce ll wall penicillin streptomyc in HEPES metrizamide leupeptin pepstatin A aprotinin phenylmethylsulfonyl fluoride (PMSF) and thiazolyl blue dye (MTT) were purchas ed from Sigma Chemic al Co.

Cell Type(s)
mast