Technical Reference #570

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom 35-mm dishes (Mat-Tek Inc., Ashland, MA, U.S.A.)

570.

4-Hydroxynonenal Induces Oxidative Stress And Death Of Cultured Spinal Cord Neurons Andrzej Malecki, Rosario Garrido, Mark P. Mattson, Bernhard Hennig, and Michal Toborek, University of Kentucky, Journal of Neurochemistry, 6, 2278, (2003)
Link To Paper

Abstract:
Primary spinal cord trauma can trigger a cascade of secondary processes leading to delayed andamplified injury to spinal cord neurons. Release of fattyacids, in particular arachidonic acid, from cell membranesis believed to contribute significantly

Keywords:
Arachidonic acid, Lipid peroxidation, Oxidative stress, Spinal cord trauma, N-Acetylcysteine, Ebselen

Materials & Methods:
Immunocytochemistry was performed to measure HNE formation in cultured spinal cord neurons exposed to arachidonic acid according to the procedure described earlier (Herbst et al., 1999). In brief, cultures of spinal cord neurons were prepared on glass

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Myocytes