569 |
Mutations
Of A Residue Within The Polyproline-Rich Region Of Env Alter The Replication Rate And Level
Of Cytopathic Effects In Chimeric Avian Retroviral Vectors |
Kevin
W. Chang, Eugene V. Barsov, Andrea L. Ferris, and Stephen H. Hughes |
mutation,
Cytopathic Effects, avian sarcoma/leukosis virus (ASLV)-based RCASBP vectors, replication, chimeric
viruses, calcium |
DF-1 cells were infected with
the RCASBP M2C
(4070A) virus generated by transfection, as described for the Southern blot
experiments. After passage 1 or at 2 days postinfection, 3 105 cells were seeded
onto 35-mm glass-bottom culture dishes (Mat-Tek
|
inverted
laser confocal microscopy |
|
|
DF-1 |
