Technical Reference #567

567.

The Extracellular Domains Of Fasl And Fas Are Sufficient For The Formation Of Supramolecular Fasl-Fas Clusters Of High Stability Frank Henkler; Eva Behrle; Kevin M. Dennehy; Andreas Wicovsky; Nathalie Peters; Clemens Warnke; Klaus Pfizenmaier; and Harald Wajant, University of Stuttgart, The Journal of Cell Biology, 268(567), (2005)
Link To Paper

Abstract:
Using fluorescent variants of Fas and FasL we show that membrane FasL and Fas form supramolecularclusters that are of flexible shape but neverthelessstable and persistent. Membrane FasL-induced Fas clusterswere formed in caspase-8– or FADD-defici

Keywords:
supramolecular FasL-Fas clusters; cluster formation; methyl--cyclodextrin; apoptosis; FADD recruitment

Materials & Methods:
5 106 HeLa cells were transfected with 20 g of plasmid DNA encoding Fas-YFP or Fas cyt-YFP by electroporation and cultured in 6 well plates containing one 18 18 cm glass cover slide per well. In parallel 2 107 HEK293 cells were transiently tra

Microscopic Technique
Fluorescence Microscopy, Live Cell

Cell Type(s)
HeLa