Technical Reference #566

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm-diameter cell culture dishes with a grided coverslip attached to the bottom (MaTek; Ashland; MA)

566.

Gentamicin Traffics Rapidly And Directly To The Golgi Complex In Llc-Pk1 Cells RUBEN M. SANDOVAL; KENNETH W. DUNN; AND BRUCE A. MOLITORIS, Indiana State University, American Journal of Physiology: Renal Physiology, 279(566), (2000)
Link To Paper

Abstract:
To study the intracellular mechanisms of aminoglycoside toxicity we used a 1:1 fluorescentconjugate of Texas Red and gentamicin (TRG) to quantifyearly uptake dynamics in renal epithelial (LLC-PK1) cells.Utilizing a protocol that quenches TRG fluor

Keywords:
aminoglycosides; lysosomal-fluorescence quenching; nephrotoxicity

Materials & Methods:
Porcine kidney proximal tubule cells (LLC-PK1 ATCC Rockville MD) were grown on 18-mm-diameter coverslips (Fisher Scientific Itasca IL) for quantitative uptake experiments or 35-mm-diameter cell culture dishes with a grided coverslip attached to t

Microscopic Technique
Confocal Microscopy, Transmission Electron

Cell Type(s)
LLC-PK