Technical Reference #565

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
5 mm diameter poly-L-lysine-coated glass-bottomed microwell dishes (Matek Corp.; Ashland; MA)

565.

Nuclear Import Of Hiv-1 Intracellular Reverse Transcription Complexes Is Mediated By Importin 7 Ariberto Fassati; Dirk GoÈ rlich; Ian Harrison; Lyubov Zaytseva and JoseÂ-Manuel Mingot, University College, The EMBO Journal, 22(565), (2003)
Link To Paper

Abstract:
Human immunode®ciency virus type 1 (HIV-1) likeother lentiviruses can infect non-dividing cells. Thisproperty depends on the active nuclear import of itsintracellular reverse transcription complex (RTC).We have studied nuclear import of puri®ed

Keywords:
HIV-1; importins; nuclear import; reverse transcription complex; small interfering RNA

Materials & Methods:
HeLa and 293T cells were grown in Dulbecco's modi®ed Eagle's medium (Gibco Laboratories Paisley UK) supplemented with 10% fetal calf serum (Helena Bioscience Newcastle UK) and 2 mM glutamine at 37°C in a humidi®ed atmosphere containing 5% CO2. Huma

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa