Technical Reference #564

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
1.4-mm coverslip inserts of Matek plates (300 cells/mm2)

564.

Lateral Membrane Biogenesis In Human Bronchial Epithelial Cells Requires 190-Kda Ankyrin-G Krishnakumar Kizhatil and Vann Bennett, HHMI, The The Journal of Biological Chemistry, 279(564), (2004)
Link To Paper

Abstract:
Ankyrin-G polypeptides are required for restriction of voltage-gated sodium channels L1 cell adhesion moleculesand IV spectrin to axon initial segments and arebelieved to couple the Na/K-ATPase to the spectrin-actinnetwork at the lateral membra

Keywords:
190-kDa Ankyrin-G; lateral plasma membrane; mitosis; ankyrin

Materials & Methods:
Human bronchial epithelial cells were a gift of Dr. Peter Mohler. The cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma). Cells were trypsinized and 60000 cells were plated on 1.4-mm

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Bronchial epithelial