Technical Reference #563

563.

Replication Of Cryptococcus Neoformans In Macrophages Is Accompanied By Phagosomal Permeabilization And Accumulation Of Vesiclescontaining Polysaccharide In The Cytoplasm Stephanie C. Tucker and Arturo Casadevall, Albert Einstein College of Medicine of Yeshiva University, PNAS, 99(563), (2002)
Link To Paper

Abstract:
Cryptococcus neoformans (CN) an encapsulated ubiquitous environmentalyeast is pathogenic for humans primarily those withcompromised immune function. CN is believed to be a facultativeintracellular pathogen. Time-lapsed video microscopy revealed

Keywords:
replication; phagosomal permeabilization; Cryptococcus neoformans; phagosome

Materials & Methods:
Infected or treated cells [where infections or treatments were performed on glass coverslips in MaTek (Ashland MA)-brand dishes] were washed with PBS to remove trace serummedium. Monolayers were fixed with 3.7% formaldehyde in 1Fix Buffer pH 7.2 (

Microscopic Technique
Video Microscopy, Time Lapse, Laser scanning confocal microscopy

Cell Type(s)
J774.16