Technical Reference #561

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-L-lysine-coated glass bottom dish (Matek Corp.)

561.

Apical Localization Of A Functional Trpc3/Trpc6-Ca2-Signaling Complex In Polarized Epithelial Cells Bidhan C. Bandyopadhyay, William D. Swaim, Xibao Liu, Robert S. Redman, Randen L. Patterson, and Indu S. Ambudkar, National Institutes of Health, The Journal of Biological Chemistry, 280(13), 12910, (2005)
Link To Paper

Abstract:
Receptor-coupled [Ca2 ]i increase is initiated in theapical region of epithelial cells and has been associatedwith apically localized Ca2 -signaling proteins. However,localization of Ca2 channels that are regulated bysuch Ca2-signaling events ha

Keywords:
apically localized Ca2-signaling proteins, TRPC channels, plasma membrane receptors

Materials & Methods:
Submandibular glands were removed and placed in ice-cold external solution with 0.02% soybean trypsin inhibitor and 0.1% bovine serum albumin. Each gland was cleaned and finely minced. The minced tissue was transferred to 8 ml of external solution cont

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK