Technical Reference #561

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-L-lysine-coated glass bottom dish (Matek Corp.)

561.

Apical Localization Of A Functional Trpc3/Trpc6-Ca2-Signaling Complex In Polarized Epithelial Cells Bidhan C. Bandyopadhyay; William D. Swaim; Xibao Liu; Robert S. Redman; Randen L. Patterson; and Indu S. Ambudkar, National Institutes of Health, The Journal of Biological Chemistry, 280(561), (2005)
Link To Paper

Abstract:
Receptor-coupled [Ca2 ]i increase is initiated in theapical region of epithelial cells and has been associatedwith apically localized Ca2 -signaling proteins. Howeverlocalization of Ca2 channels that are regulated bysuch Ca2-signaling events ha

Keywords:
apically localized Ca2-signaling proteins; TRPC channels; plasma membrane receptors

Materials & Methods:
Submandibular glands were removed and placed in ice-cold external solution with 0.02% soybean trypsin inhibitor and 0.1% bovine serum albumin. Each gland was cleaned and finely minced. The minced tissue was transferred to 8 ml of external solution cont

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK