Technical Reference #561
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
poly-L-lysine-coated glass bottom dish (Matek Corp.) 
561. |
Apical Localization Of A Functional Trpc3/Trpc6-Ca2-Signaling Complex In Polarized Epithelial Cells
Bidhan C. Bandyopadhyay; William D. Swaim; Xibao Liu; Robert S. Redman;
Randen L. Patterson; and Indu S. Ambudkar,
National Institutes of Health,
The Journal of Biological Chemistry,
280(561),
(2005)
Link To Paper
Abstract:
Receptor-coupled [Ca2 ]i increase is initiated in theapical region of epithelial cells and has been associatedwith apically localized Ca2 -signaling proteins. Howeverlocalization of Ca2 channels that are regulated bysuch Ca2-signaling events ha Keywords:
apically localized Ca2-signaling proteins; TRPC channels; plasma membrane receptors Materials & Methods:
Submandibular glands were removed and placed in ice-cold
external solution with 0.02% soybean trypsin inhibitor and 0.1% bovine
serum albumin. Each gland was cleaned and finely minced. The minced
tissue was transferred to 8 ml of external solution cont Microscopic Technique
Confocal Microscopy Cell Type(s)
MDCK |