Technical Reference #557

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-L-lysine-coated 35 mm glass-bottomed culture dish (MattTek Corp.; Ashland MA)

557.

Live Dynamics Of Dictyostelium Cofilin Suggests A Role In Remodeling Actin Latticework Into Bundles Hiroyuki Aizawa; Yoshio Fukui and Ichiro Yahara, The Tokyo Metropolitan Institute of Medical Science, The Journal of Cell Science, 110(557), (1997)
Link To Paper

Abstract:
Cofilin an indispensable actin-regulating protein represents the ‘cofilin family’ of actin-binding proteins existingin a wide variety of organisms. Our previous and other invitro studies have implied that cofilin can accelerate transformationof f

Keywords:
Actin; Cell movement; Cofilin; Dictyostelium; Green fluorescent protein

Materials & Methods:
The live cells were observed under an Olympus IMT-2 inverted microscope using an ´100 plan apochromat objective lens (S-Plan Apo NA 1.4). The microscope was attached to a real time laser confocal microscope system ‘Insight Plus-IQ’ (Meridian Instrumen

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Dictyostelium discoideum