Technical Reference #555

555.

Baf Is Required For Emerin Assembly Into The Reforming Nuclear Envelope Tokuko Haraguchi; Takako Koujin; Miriam Segura-Totten; Kenneth K. Lee; Yosuke Matsuoka; Yoshihiro Yoneda; Katherine L. Wilson and Yasushi Hiraoka, Japan Science and Technology Corporation, The Journal of Cell Science, 114(555), (2001)
Link To Paper

Abstract:
Mutations in emerin cause the X-linked recessive form of Emery-Dreifuss muscular dystrophy (EDMD). Emerinlocalizes at the inner membrane of the nuclear envelope(NE) during interphase and diffuses into the ER whenthe NE disassembles during mitosis.

Keywords:
Nuclear envelope; Lamin A; Lamin-associated polypeptide 2; MAN1; Emery-Dreifuss muscular dystrophy; Barrierto- autointegration factor; Chromosome

Materials & Methods:
Cells were grown in a glass-bottom culture dish (MatTech USA). GFP fusion plasmids (1 mg) were transfected into cells with LipofectaminePlus (Gibco BRL) according to manufacturer’s methods except that the incubation time with DNA was reduced to 1.5 h

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
HeLa