Technical Reference #553

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-l-lysine-coated culture glass-bottomed microwell dishes (Mat Tec; Ashland; MA; USA)

553.

Increase In Intracellular Ca2+ Concentration Is Not The Only Cause Of Lidocaine-Induced Cell Damage In The Cultured Neurons Oflymnaea Stagnalis Toshiharu Kasaba; Shin Onizuka; Masatoshi Kashiwada; and Mayumi Takasaki, University of Miyazaski, Journal of Anesthesia, 20(553), (2006)
Link To Paper

Abstract:
To determine whether the increase in intracellular Ca2+ concentration induced by lidocaine produces neurotoxicitywe compared morphological changes and Ca2+ concentrationsusing fura-2 imaging in the cultured neurons ofLymnaea stagnalis. We used B

Keywords:
Lidocaine · Neurotoxicity · Ca2+ concentration

Materials & Methods:
Laboratory-raised stocks of the freshwater snail Lymnaea stagnalis at 3 to 6 months of age with a shell length of 10–25mm were used. Lymnaea normal saline contained 51.3mM NaCl 1.7mM KCl 4.1mM CaCl2 1.5mM MgCl2 and 5.0mM N-2-hydroxyethylpiper

Microscopic Technique
Optical Microscopy

Cell Type(s)
Lymnaea