Technical Reference #552

552.

Suicide Gene Therapy Using Adenovirus Vector For Human Oral Squamous Carcinoma Cell Line In Vitro NORIYUKI YAMAMOTO; YASUSHI HAYASHI; HIDEAKI KAGAMI; TAKAFUMI FUKUI; HIROKAZU FUKUHARA; IWAI TOHNAI; MINORU UEDA; MASAAKI MIZUNO and JUN YOSHIDA, Nagoya University, Nagoya Journal of Medical Science, 67(552), (2005)
Link To Paper

Abstract:
Recently suicide gene therapy using the herpes simplex virus thymidine kinase (HSVtk) gene followedby ganciclovir (GCV) administration was evaluated for the treatment of cancer. The purpose of this studywas to investigate the effectiveness of suicide

Keywords:
Squamous Cell Carcinoma; Adenovirus; Herpes Simplex Virus Thymidine Kinase Gene; Ganciclovir; Bystander effect

Materials & Methods:
We plated 2×104 cells/ml of the SAS line into a glass bottomed culture dish (Mat Tec Corporation USA) and incubated a 5% CO2-incubator at 37°C for 24 hours. Then the medium was changed the cells were infected with AxCAHSVtk vectors at an MOI of 10 an

Microscopic Technique
Video Enhanced Contrast Differential Interference Contrast (VEC-DIC)

Cell Type(s)
HEK-293