Technical Reference #548

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
30-mm Petri dishes with glass coverslip bottoms (MatTec)

548.

The Intracellular Translocation Of The Components Of The Fibroblast Growth Factor 1 Release Complex Precedes Their Assembly Prior To Export Igor Prudovsky; Cinzia Bagala; Francesca Tarantini; Anna Mandinova; Raffaella Soldi; Stephen Bellum; and Thomas Maciag, Maine Medical Cancer Research Institute, The Journal of Cell Biology, 158(548), (2002)
Link To Paper

Abstract:
The release of signal peptideless proteins occursthrough nonclassical export pathways and the releaseof fibroblast growth factor (FGF)1 in response tocellular stress is well documented. Although biochemicalevidence suggests that the formation of a

Keywords:
fibroblast growth factor 1; heat shock; amlexanox; F-actin

Materials & Methods:
The real-time in vivo observations were performed using FGF1:GFP NIH 3T3 cell transfectants plated on fibronectin-coated (10 g/cm 2 ) 30- mm Petri dishes with glass coverslip bottoms (MatTec) seeded at 10 5 cells/ dish. After 24 h the medium w

Microscopic Technique
Confocal Microscopy

Cell Type(s)
3T3