Technical Reference #545

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom 35-mm dishes (Mat-Tek, Ashland, MA)

545.

Caspase-Mediated Cleavage Of The Ca2/Calmodulin-Dependent Protein Kinase-Like Kinase Facilitates Neuronal Apoptosis Marieke Kruidering, Theo Schouten, Gerard I. Evan, and Erno Vreugdenhil, Leiden University, The Journal of Biological Chemistry, 21(3), 38419, (2005)
Link To Paper

Abstract:
This study was designed to identify the role of a recentlyidentified Ca2 /calmodulin-dependent protein kinase(CaMK)-like kinase (CaMKLK) in neuronal apoptosis.For this purpose, we studied proteolytic cleavageof CaMKLK by caspases in vitro and in n

Keywords:
Neuronal Apoptosis, proteolytic cleavage, kinase, caspases

Materials & Methods:
Cells were seeded on glass-bottomed coverslip dishes (Matteck Corp., Ashland, OR) 24�48 h prior to injection. Nuclear microinjection was performed using an automated microinjection system (Eppendorf Transjector 5246, micromanipulator 5171). Identical

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
mast