Technical Reference #544
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
35 mm glass-bottom Petri dish (MatTeck Corp.) 
544. |
Contrasting Effects Of Basic Fibroblast Growth Factor And Neurotrophin 3 On Cell Cycle Kinetics Of Mouse Cortical Stem Cells
Agnes Lukaszewicz, Pierre Savatier, VeŽ ronique Cortay, Henry Kennedy, and Colette Dehay,
Institut National de la Sante et de la Recherche Medicale,
The Journal of Neuroscience,
66(6),
6614,
(2004)
Link To Paper
Abstract:
Basic fibroblast growth factor (bFGF) exerts a mitogenic effecton cortical neuroblasts, whereas neurotrophin 3 (NT3) promotesdifferentiation in these cells. Here we provide evidencethat both the mitogenic effect of bFGF and the differentiationpromot Keywords:
G1 phase; cell cycle; proliferation; neuroblast;
time-lapse videomicroscopy; bFGF; NT3; corticogenesis Materials & Methods:
Cortical precursors from green fluorescent protein (GFP; Okabe et al.,
1997) / and / mice were seeded on poly-L-lysine- and laminin-coated glass coverslips to obtain 25% of GFP cells. This low proportion
of GFP cells makes it possible to reliably Microscopic Technique
Video Microscopy, Time Lapse Cell Type(s)
Cortical |
