Technical Reference #544

544.

Contrasting Effects Of Basic Fibroblast Growth Factor And Neurotrophin 3 On Cell Cycle Kinetics Of Mouse Cortical Stem Cells Agnes Lukaszewicz; Pierre Savatier; Ve´ ronique Cortay; Henry Kennedy; and Colette Dehay, Institut National de la Sante et de la Recherche Medicale, The Journal of Neuroscience, 66(544), (2004)
Link To Paper

Abstract:
Basic fibroblast growth factor (bFGF) exerts a mitogenic effecton cortical neuroblasts whereas neurotrophin 3 (NT3) promotesdifferentiation in these cells. Here we provide evidencethat both the mitogenic effect of bFGF and the differentiationpromot

Keywords:
G1 phase; cell cycle; proliferation; neuroblast; time-lapse videomicroscopy; bFGF; NT3; corticogenesis

Materials & Methods:
Cortical precursors from green fluorescent protein (GFP; Okabe et al. 1997) / and / mice were seeded on poly-L-lysine- and laminin-coated glass coverslips to obtain 25% of GFP cells. This low proportion of GFP cells makes it possible to reliably

Microscopic Technique
Video Microscopy, Time Lapse

Cell Type(s)
Cortical