Technical Reference #543

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass dishes (Mat Tek)

543.

Activation Of Rac1 By A Crk Sh3-Binding Protein; Dock180 Etsuko Kiyokawa; Yuko Hashimoto; Shin Kobayashi; Haruhiko Sugimura; Takeshi Kurata; and Michiyuki Matsuda, National Institute of Infectious Diseases, Genes & Development, 12(543), (1998)
Link To Paper

Abstract:
DOCK180 is involved in integrin signaling through rkII-p130Cas complexes. We have studied the involvementof DOCK180 in Rac1 signaling cascades.DOCK180 activated JNK in a manner dependent onRac1 Cdc42Hs and SEK and overexpression ofDOCK180 inc

Keywords:
DOCK180; actin cytoskeleton; Rho-family proteins; CrkII

Materials & Methods:
NIH-3T3 cells were plated subconfluently on fibronectin-coated glass dishes (Mat Tek) 16 hr before mincroinjection. Expression plasmids were injected into the nucleus by use of an Automated Microinjection System (Carl Zeiss). After 16 hr cells were fi

Microscopic Technique
Confocal Microscopy

Cell Type(s)
3T3