Technical Reference #540

540.

Different Populations Of Rna Polymerase Ii In Living Mammalian Cells Miki Hieda; Henry Winstanley; Philip Maini; Francisco J. Iborra & Peter R. Cook, University of Oxford, Chromosome Research, 13(540), (2005)
Link To Paper

Abstract:
RNA polymerase II is responsible for transcription of most eukaryotic genes but despite exhaustiveanalysis little is known about how it transcribes natural templates in vivo. We studied polymerase dynamicsin living Chinese hamster ovary cells using

Keywords:
DRB; FLIP; heat shock; photobleaching; RNA polymerase II

Materials & Methods:
Cells were grown in glass-bottomed microwell dishes (Mat Tek MA) for 40–48 h to 50% confluence. The standard FLIP experiment in Figure 1 was performed as described by Kimura et al. (2002) using a Radiance 2000 confocal microscope (488-nm-laser line;

Microscopic Technique
Confocal Microscopy

Cell Type(s)
tsTM4