Technical Reference #539

539.

Novel Follicular Dendritic Cell Molecule; 8D6; Collaborates With Cd44 Insupporting Lymphomagenesis By A Burkitt Lymphoma Cell Line; L3055 Li Li; Sun-Ok Yoon; Dan-Dan Fu; Xin Zhang; and Yong Sung Choi, Ochsner Clinic Foundation, Blood, 104(539), (2004)
Link To Paper

Abstract:
The lymphoid follicle is a specialized microenvironmentfor the differentiation ofantigen (Ag)–activated B cells; the majorstromal cell components in lymphoid follicleare the follicular dendritic cells(FDCs). At the same time most of theB-cell

Keywords:
lymphomagenesis; lymphoid follicle; follicular dendritic cells; L3055; monoclonal antibodies; B-cell lymphomas

Materials & Methods:
HK cells (1.5 105 cells per dish) were seeded 1 day before transfection on glass-bottom culture dishes (35 mm MatTek Ashland MA) to obtain 70% to 80% confluence. Cells were transfected with CD44-GFP construct using Effectene Transfection Reagent (

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HK