Technical Reference #538

538.

Dishevelled (Dvl-2) Activates Canonical Wnt Signaling In The Absence Of Cytoplasmic Puncta Matthew J. Smalley; Nathalie Signoret; David Robertson; Alan Tilley; Anthony Hann ; Ken Ewan; Yanning Ding; Hugh Paterson and Trevor C. Dale, The Institute of Cancer Research, The Journal of Cell Science, 118(538), (2005)
Link To Paper

Abstract:
Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressedmammalian Dishevelled 2 (Dvl-2) activates downstreamsignalling and localises to cytoplasmic puncta. It has beensuggested that these Dvl-2-con

Keywords:
Dishevelled; Dvl-2; endosomal; vesicle; Wnt

Materials & Methods:
Groups of subconfluent MDCK cells cultured on 50 mm MatTek glass-bottomed Petri dishes were microinjected in the nucleus with HA-Dvl-2-EGFP plasmid diluted to 25 g/ml in PBS. After a 6 hour expression period the HA-Dvl-2-EGFP expressing cells were im

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
3T3