Technical Reference #537

537.

Carboxy Terminus Of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization; Transcriptionalrepression; And Targeting To Nuclear Bodies DAVID R. SCHWAM; RANDY L. LUCIANO; SHAHANA S. MAHAJAN; LAIYEE WONG; AND ANGUS C. WILSON, New York University School of Medicine, The Journal of Virology, 74(537), (2000)
Link To Paper

Abstract:
Human herpesvirus 8 (HHV-8; also known as Kaposi’s sarcoma-associated herpesvirus) is the causativeagent of Kaposi’s sarcoma and certain B-cell lymphomas. In most infected cells HHV-8 establishes a latentinfection characterized by the expression of l

Keywords:
Human herpesvirus 8; Dimerization; Transcriptional Repression; Kaposi’s sarcoma; B-cell lymphomas; Epstein-Barr virus; latency-associated nuclear antigen (LANA)

Materials & Methods:
Cos-1 cells were plated into 35-mm dishes containing a glass coverslip-covered 15-mm cutout (MatTek Corporation) and transfected the next day with 500 ng of GFP expression plasmid. Cells were examined 24 h posttransfection with a Zeiss Axiovert epifluo

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293