Technical Reference #537

537.

Carboxy Terminus Of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization, Transcriptionalrepression, And Targeting To Nuclear Bodies DAVID R. SCHWAM, RANDY L. LUCIANO, SHAHANA S. MAHAJAN, LAIYEE WONG, AND ANGUS C. WILSON, New York University School of Medicine, The Journal of Virology, 74(18), 8533, (2000)
Link To Paper

Abstract:
Human herpesvirus 8 (HHV-8; also known as Kaposi’s sarcoma-associated herpesvirus) is the causativeagent of Kaposi’s sarcoma and certain B-cell lymphomas. In most infected cells, HHV-8 establishes a latentinfection characterized by the expression of l

Keywords:
Human herpesvirus 8, Dimerization, Transcriptional Repression, Kaposi’s sarcoma, B-cell lymphomas, Epstein-Barr virus, latency-associated nuclear antigen (LANA)

Materials & Methods:
Cos-1 cells were plated into 35-mm dishes containing a glass coverslip-covered 15-mm cutout (MatTek Corporation) and transfected the next day with 500 ng of GFP expression plasmid. Cells were examined 24 h posttransfection with a Zeiss Axiovert epifluo

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293