Technical Reference #536

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom microwell dishes (MatTek Corporation; Ashland; USA)

536.

Partial Cell Fusion: A Newly Recognized Type Of Communication Between Dedifferentiating Cardiomyocytes And Fibroblasts Ronald B. Driesen; Gerrit D. Dispersyn; Fons K. Verheyen; Stefan M. van den Eijnde; Leo Hofstra; Fred Thone; Petra Dijkstra; Wiel Debie; Marcel Borgers; Frans C.S. Ramaekers, University of Maastricht, Cardiovascular Research, 68(536), (2005)
Link To Paper

Abstract:
Fibroblasts have been shown to couple to neonatal cardiomyocytes in heterocellular cultures through functional gap junctions.Our objective was to provide evidence for an additional type of heterocellular communication between fibroblasts and adult cardi

Keywords:
Cell communication; Cardiomyocytes; Fibroblasts; Gap junctions; Partial cell fusion; Annexin V

Materials & Methods:
New Zealand white rabbits (ca. 2 kg) were used for this study. Cardiomyocytes and cardiac fibroblasts were isolated from adult rabbits by a retrograde collagenase perfusion as previously described [11]. In brief rabbits were anaesthetized by intra-art

Microscopic Technique
Video Microscopy, Time Lapse

Cell Type(s)
HEK-293