Technical Reference #535

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom petri dish (MatTek Corp, Ashland, MD)

535.

Claudin 14 Knockout Mice, A Model For Autosomal Recessive Deafness Dfnb29, Are Deaf Due To Cochlear Hair Cell Degeneration Tamar Ben-Yosef, Inna A. Belyantseva, Thomas L. Saunders, Elizabeth D. Hughes, Kohei Kawamoto, Christina M. Van Itallie, Lisa A. Beyer, Ka¨rin Halsey, Donald J. Gardner, Edward R. Wilcox, Julia Rasmussen, James M. Anderson, David F. Dolan, Andrew Forge, National Institutes of Health, Human Molecular Genetics, 12(16), 2059, (2003)
Link To Paper

Abstract:
Tight junctions (TJs) create ion-selective paracellular permeability barriers between extracellular compartments.In the organ of Corti of the inner ear, TJs of the reticular lamina separate Kþ-rich endolymph and Naþrichperilymph. In humans, mutations

Keywords:
autosomal recessive deafness DFNB29, cochlear hair cell degeneration, Tight junctions, inner ear, Cldn14, TJ strands

Materials & Methods:
OC cultures were prepared from cochleae of Cldn14 /, Cldn14þ/ and Cldn14þ/þ mice at P3–P5 according to the procedure described (87) with some modifications. The cochleae were dissected in Leibowitz cell culture medium, L-15 (Invitrogen, Carlsbad, CA

Microscopic Technique
Electron Microscopy, Transmission Electron, Indirect Immunofluorescence Microscopy

Cell Type(s)
MDCK