Technical Reference #535

535.

Claudin 14 Knockout Mice; A Model For Autosomal Recessive Deafness Dfnb29; Are Deaf Due To Cochlear Hair Cell Degeneration Tamar Ben-Yosef; Inna A. Belyantseva; Thomas L. Saunders; Elizabeth D. Hughes; Kohei Kawamoto; Christina M. Van Itallie; Lisa A. Beyer; Ka¨rin Halsey; Donald J. Gardner; Edward R. Wilcox; Julia Rasmussen; James M. Anderson; David F. Dolan; Andrew Forge, National Institutes of Health, Human Molecular Genetics, 12(535), (2003)
Link To Paper

Abstract:
Tight junctions (TJs) create ion-selective paracellular permeability barriers between extracellular compartments.In the organ of Corti of the inner ear TJs of the reticular lamina separate Kþ-rich endolymph and Naþrichperilymph. In humans mutations

Keywords:
autosomal recessive deafness DFNB29; cochlear hair cell degeneration; Tight junctions; inner ear; Cldn14; TJ strands

Materials & Methods:
OC cultures were prepared from cochleae of Cldn14 / Cldn14þ/ and Cldn14þ/þ mice at P3–P5 according to the procedure described (87) with some modifications. The cochleae were dissected in Leibowitz cell culture medium L-15 (Invitrogen Carlsbad CA

Microscopic Technique
Electron Microscopy, Transmission Electron, Indirect Immunofluorescence Microscopy

Cell Type(s)
MDCK