Technical Reference #533

533.

Visualization Of Local Ca2+ Dynamics With Genetically Encoded Bioluminescent Reporters Kelly L. Rogers; Jacques Stinnakre; Cendra Agulhon; Delphine Jublot; Spencer L. Shorte; Eric J. Kremer and Philippe Brulet, Institut Pasteur, European Journal of Neuroscience, 21(533), (2005)
Link To Paper

Abstract:
Measurements of local Ca2+ signalling at different developmental stages and / or in specific cell types is important for understandingaspects of brain functioning. The use of light excitation in fluorescence imaging can cause phototoxicity photobleachi

Keywords:
bioluminescence imaging; Ca2+-dependent neural activity; Ca2+ microdomains

Materials & Methods:
COS7 and neuroblastoma cells (Neuro2A mouse) were grown in DMEM supplemented with 10% FCS 2 mm glutamine and 50 units / mL of penicillin / streptomycin (Invitrogen life technologies). Cultures were incubated at 37 !C in a humidified atmosphere co

Microscopic Technique
Confocal Microscopy, Laser Scanning, Inverted Microscopy

Cell Type(s)
COS-7