533 |
Visualization
Of Local Ca2+ Dynamics With Genetically Encoded Bioluminescent Reporters |
Kelly
L. Rogers, Jacques Stinnakre, Cendra Agulhon, Delphine Jublot, Spencer L. Shorte, Eric J. Kremer
and Philippe Brulet |
bioluminescence
imaging, Ca2+-dependent neural activity, Ca2+ microdomains |
COS7 and neuroblastoma cells
(Neuro2A, mouse), were grown in
DMEM supplemented with 10% FCS, 2 mm glutamine and
50 units / mL of penicillin / streptomycin (Invitrogen, life technologies).
Cultures were incubated at 37 !C, in a humidified atmosphere
co
|
laser
scanning confocal microscopy, inverted microscopy |
MONKEY,
AFRICAN GREEN |
fibroblast-like |
COS-7 |
