Technical Reference #532

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-L-lysine-coated petri dishes with glass bottoms (MatTek, Ashland, MA)

532.

Alterations In Receptor Expression Or Agonist Concentration Change The Pathways Gastrin-Releasing Peptide Receptoruses To Regulate Extracellular Signal-Regulated Kinase Pei-Wen Chen and Glenn S. Kroog, Albert Einstein College of Medicine of Yeshiva University, Molecular Pharmacology, 66(6), 1627, (2004)
Link To Paper

Abstract:
G protein-coupled receptors activate extracellular signalregulated kinases (ERKs) via different pathways in differentcell types. In this study, we demonstrate that gastrin-releasingpeptide receptor (GRPr) regulates ERK through multiplepathways in a

Keywords:
Receptor Expression, Agonist Concentration, Gastrin-Releasing Peptide Receptor, extracellular signalregulated kinases, Mitogen-activated protein kinases,

Materials & Methods:
Myc-GRPr cells were plated in glass bottom 35-mm dishes (Mat- Tek, Ashland, MA) at 150,000 cells/dish and cultured overnight. Cells were transfected with 0.3 g of pEGFP-N1-arrestin2 or arrestin3- GFP by LipofectAMINE method following the manufacturer’

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Myc-GRPr