Technical Reference #532
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
poly-L-lysine-coated petri dishes with glass bottoms (MatTek, Ashland, MA) 
532. |
Alterations In Receptor Expression Or Agonist Concentration Change The Pathways Gastrin-Releasing Peptide Receptoruses To Regulate Extracellular Signal-Regulated Kinase
Pei-Wen Chen and Glenn S. Kroog,
Albert Einstein College of Medicine of Yeshiva University,
Molecular Pharmacology,
66(6),
1627,
(2004)
Link To Paper
Abstract:
G protein-coupled receptors activate extracellular signalregulated kinases (ERKs) via different pathways in differentcell types. In this study, we demonstrate that gastrin-releasingpeptide receptor (GRPr) regulates ERK through multiplepathways in a Keywords:
Receptor Expression, Agonist Concentration, Gastrin-Releasing Peptide Receptor, extracellular signalregulated
kinases, Mitogen-activated protein kinases, Materials & Methods:
Myc-GRPr cells were plated in glass bottom 35-mm dishes (Mat-
Tek, Ashland, MA) at 150,000 cells/dish and cultured overnight.
Cells were transfected with 0.3 g of pEGFP-N1-arrestin2 or arrestin3-
GFP by LipofectAMINE method following the manufacturer’ Microscopic Technique
Confocal Microscopy Cell Type(s)
Myc-GRPr |
