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Technical Reference #531

Glass Bottom Culture Dishes

This study used MatTek product(s):

P35GC-1.5-14-C

Citation in paper containing MatTek reference:
poly-L-lysine-coated petri dishes with glass bottoms (MatTek; Ashland; MA)

531.

In Vitro Activation Of Murine Drg Neurons By Cgrp-Mediated Mucosal Mastcell Degranulation F. De Jonge; A. De Laet; L. Van Nassauw; J. K. Brown; H. R. P. Miller; P.-P. van Bogaert J.-P. Timmermans; and A. B. A. Kroese, University of Antwerp, American Journal of Physiology: Gastrointestinal and Liver Physiology, 287(531), (2004)
Link To Paper

Abstract:
Upregulation of CGRP-immunoreactive (IR) primary afferent nerve fibers accompanied by mastocytosisis characteristic for the Schistosoma mansoni-infected murineileum. These mucosal mast cells (MMC) and CGRP-IR fibers whichoriginate from dorsal root

Keywords:
calcium imaging; electrophysiology; cultured mucosal mast cells; cultured dorsal root ganglia neurons; mast cell juice

Materials & Methods:
Adult mouse DRG neurons were isolated as previously described (33). First animals were anesthetized by an overdose of ether and bled from the abdominal aorta. Next the spinal cord was isolated and the ganglia dissected using microforceps. After remo

Microscopic Technique
Confocal Microscopy, Transmission Electron

Cell Type(s)
Dorsal root ganglion, DRG