531 |
In
Vitro Activation Of Murine Drg Neurons By Cgrp-Mediated Mucosal Mastcell Degranulation |
F.
De Jonge, A. De Laet, L. Van Nassauw, J. K. Brown, H. R. P. Miller,
P.-P. van Bogaert J.-P. Timmermans, and A. B. A. Kroese |
calcium
imaging; electrophysiology; cultured mucosal mast cells;
cultured dorsal root ganglia neurons; mast cell juice |
Adult mouse DRG neurons were
isolated as
previously described (33). First, animals were anesthetized by an
overdose of ether and bled from the abdominal aorta. Next, the spinal
cord was isolated and the ganglia dissected using microforceps. After
remo
|
transmission
electron microscopy, confocal laser scanning microscopy |
MOUSE |
neuronal |
DRG |