Technical Reference #530

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysine-coated; glass-bottomed dish (MatTek; Ashland; MA)

530.

Mitochondrial Metabolism Reveals A Functional Architecture In Intact Islets Of Langerhans From Normal And Diabetic Psammomys Obesus S. M. Katzman; M. A. Messerli; D. T. Barry; A. Grossman; T. Harel; J. D. Wikstrom; B. E. Corkey; P. J. S. Smith; and O. S. Shirihai, Tufts University, American Journal of Physiology: Endocrinology and Metabolism, 287(530), (2004)
Link To Paper

Abstract:
The cells within the intact islet ofLangerhans function as a metabolic syncytium secreting insulin in acoordinated and oscillatory manner in response to external fuel. Withincreased glucose the oscillatory amplitude is enhanced leading tothe hy

Keywords:
diabetes; metabolic oscillations; mitochondrial membrane potential; rhodamine 123

Materials & Methods:
Mitochondrial membrane potential was measured using the potentiometric dye Rh123 (Molecular Probes Eugene OR) loaded at a final concentration of 13 M. This concentration of dye is not harmful to the cells; in studies of isolated -cells exposure o

Microscopic Technique
Confocal Microscopy, Time Lapse

Cell Type(s)
Islet