Technical Reference #529

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
optical glass bottom of a dish (MatTek; Ashland; MA)

529.

Increased Reactive Oxygen Species Contribute To High Nacl-Induced Activationof The Osmoregulatory Transcription Factor Tonebp/Orebp Xiaoming Zhou; Joan D. Ferraris; Qi Cai; Anupam Agarwal; and Maurice B. Burg, Uniformed Services University of the Health Sciences, American Journal of Physiology: Renal Physiology, 289(529), (2005)
Link To Paper

Abstract:
activation of the transcription factor tonicity-responsive enhancer binding protein/osmotic response element binding protein (TonEBP/OREBP) remain incompletelyunderstood. High NaCl has been reported to produce oxidativestress. Reactive oxygen specie

Keywords:
superoxides; nitric oxide; ouabain; transactivation; nuclear translocation

Materials & Methods:
Dihydroethidium (DHE) enters cells freely. Superoxides oxidize DHE into ethidium or a structurally similar product which intercalates into DNA producing fluorescence (5). One to 1.2 106 cells were grown overnight on the optical glass bottom of a d

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293