Technical Reference #528

528.

Cellular Stresses Induce The Nuclear Accumulation Of Importin And Cause A Conventional Nuclear Import Block Yoichi Miyamoto, Takuya Saiwaki, Junichi Yamashita, Yoshinari Yasuda, Ippei Kotera, Satoshi Shibata, Masaki Shigeta, Yasushi Hiraoka, Tokuko Haraguchi, and Yoshihiro Yoneda, Osaka University, The Journal of Cell Biology, 165(5), 623, (2004)
Link To Paper

Abstract:
We report here that importin accumulates reversibly in the nucleus in response to cellularstresses including UV irradiation, oxidative stress,and heat shock. The nuclear accumulation of importin appears to be triggered by a collapse in the Ra

Keywords:
nuclear import block, cellular stresses, importin , Ran gradient

Materials & Methods:
Importin (mouse Rch1) and were constructed with pEGFP-C1 plasmid (CLONTECH Laboratories, Inc.). HeLa cells were plated onto a glass bottom dish (MatTek Corporation) cultured for 1–2 d before use. After the constructs were transfected using Effecten

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa