Technical Reference #528

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom dish (MatTek Corporation)

528.

Cellular Stresses Induce The Nuclear Accumulation Of Importin And Cause A Conventional Nuclear Import Block Yoichi Miyamoto; Takuya Saiwaki; Junichi Yamashita; Yoshinari Yasuda; Ippei Kotera; Satoshi Shibata; Masaki Shigeta; Yasushi Hiraoka; Tokuko Haraguchi; and Yoshihiro Yoneda, Osaka University, The Journal of Cell Biology, 165(528), (2004)
Link To Paper

Abstract:
We report here that importin accumulates reversibly in the nucleus in response to cellularstresses including UV irradiation oxidative stressand heat shock. The nuclear accumulation of importin appears to be triggered by a collapse in the Ra

Keywords:
nuclear import block; cellular stresses; importin ; Ran gradient

Materials & Methods:
Importin (mouse Rch1) and were constructed with pEGFP-C1 plasmid (CLONTECH Laboratories Inc.). HeLa cells were plated onto a glass bottom dish (MatTek Corporation) cultured for 1–2 d before use. After the constructs were transfected using Effecten

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa