Technical Reference #527
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
bottom-glass dishes (Mat Tek; Ashland; MA) 
527. |
Fluorescence Lifetime Imaging Of Nuclear Dna: Effect Of Fluorescence Resonance Energy Transfer
Shin-ichi Murata; Petr Herman; Hai-Jui Lin; and Joseph R. Lakowicz,
University of Maryland,
Cytometry,
41(527),
(2000)
Link To Paper
Abstract:
DNA fluorescence dyes have been used tostudy DNA dynamics chromatin structure and cell cycleanalysis. However most microscopic fluorescence studiesof DNA use only steady-state measurements and do nottake advantage of the additional information Keywords:
FLIM; FRET; fibroblasts; nucleus; nucleic acid;
texture analysis; Hoechst 33258 Materials & Methods:
Mouse fibroblasts (3T3-Swiss albino ATCC number
CCL-92) were grown asynchronously at 37°C and 5% CO2
in the bottom-glass dishes (Mat Tek Ashland MA) containing
Dulbecco’s modified Eagle’s medium with 10% calf
serum. Microscopic Technique
Fluorescence Microscopy, Fluorescence Intensity and Lifetime Imaging Cell Type(s)
3T3 |
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