Technical Reference #526

526.

Recycling Endosomes Can Serve As Intermediates During Transport From The Golgi To The Plasma Membrane Of Mdck Cells Agnes Lee Ang; Tomohiko Taguchi; Stephen Francis; Heike Fölsch; Lindsay J. Murrells; Marc Pypaert; Graham Warren; and Ira Mellman, Yale University, The Journal of Cell Biology, 167(526), (2004)
Link To Paper

Abstract:
The AP-1B clathrin adaptor complex is responsiblefor the polarized transport of many basolateralmembrane proteins in epithelial cells. Localizationof AP-1B to recycling endosomes (REs) along with othercomponents (exocyst subunits and Rab8) involve

Keywords:
Recycling endosomes; transport; AP-1B clathrin adaptor complex; vesicular stomatitis virus glycoprotein G

Materials & Methods:
Coverslip-grown MDCKT cells were infected with ts045 VSV-G-YFP and induced with 5 mM butyrate for 16 h at 40C. Tfn uptake was performed as described above. Cells were incubated 2 h in media at 20C (last hour in media plus 0.1 mg/ml CHX). Coverslips w

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK