Technical Reference #525

525.

Hfis1; A Novel Component Of The Mammalian Mitochondrial Fission Machinery Dominic I. James; Philippe A. Parone; Yves Mattenberger; and Jean-Claude Martinou, University of Geneva, The The Journal of Biological Chemistry, 278(525), (2003)
Link To Paper

Abstract:
The balance between the fission and fusion mechanisms regulate the morphology of mitochondria. In thisstudy we have identified a mammalian protein that wecall hFis1 which is the orthologue of the yeast Fis1pknown to participate in yeast mitochondr

Keywords:
Mitochondrial Fission; hFis1; dominant negative mutant of Drp1; apoptosis; Mitochondria

Materials & Methods:
COS-7 or HeLa cells were plated at 2.5 105 cells/3-cm glass bottomed dish (Mattek) 6 h prior to transfection. Cells were then transfected using FuGENE (Roche Applied Science) with a 1:1 ratio of target DNA to YFP-mito (Clontech) and a total of 1 g

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7