525 |
Hfis1,
A Novel Component Of The Mammalian Mitochondrial Fission Machinery |
Dominic
I. James, Philippe A. Parone, Yves Mattenberger, and Jean-Claude Martinou |
Mitochondrial
Fission, hFis1, dominant negative mutant of Drp1, apoptosis, Mitochondria |
COS-7 or HeLa cells were plated
at 2.5 105
cells/3-cm glass bottomed dish (Mattek) 6 h prior to transfection. Cells
were then transfected using FuGENE (Roche Applied Science) with a
1:1 ratio of target DNA to YFP-mito (Clontech) and a total of 1 g
|
Confocal
microscopy |
MONKEY,
AFRICAN GREEN |
fibroblast-like |
COS-7 |
